Protocol for producing a rat model of non-obese prediabetes using a mild hypercaloric diet approach

Abstract

Metabolic disease complications pose a significant health risk due to their early development, making their diagnosis and radical therapy a considerable challenge. Here, we present a protocol for producing a rat model of non-obese prediabetes characterized by hyperinsulinemia, normoglycemia, and normal body weight. We describe steps for inducing the model in Sprague-Dawley (SD) male and ovariectomized female rats by free feeding on a mild hypercaloric diet. This protocol offers a potential model of metabolically unhealthy lean individuals.

How to cite

Dwaib, H. S., & El-Yazbi, A. F. (2024). Protocol for producing a rat model of non-obese prediabetes using a mild hypercaloric diet approach. STAR protocols, 5(3), 103276.‏

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https://www.sciencedirect.com/science/article/pii/S2666166724004416